Cell Activation Cocktail (with Brefeldin A) from BioLegend

Product Specs
ItemCell Activation Cocktail (with Brefeldin A)
CompanyBioLegend
Price Pricing Info Supplier Page View Company Product Page
Catalog Number423303
Quantity100 µL
ApplicationsActivation, Flow Cytometry (FC), Intracellular Staining for Flow Cytometry (ICFC)
FormatCell Activation Cocktail (with Brefeldin A) is composed of PMA, ionomycin, and Brefeldin A in DMSO (500X)

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BioLegend

8999 BioLegend Way

San Diego, California 92121

United States

Phone: 1-877-246-5343 Toll-Free, USA & Canada
1-858-768-5800 International
Fax: 1-877-455-9587

Company Profile

Website: biolegend.com

(1) Piezo1 facilitates optimal T cell activation during tumor challengeOncoImmunologyDecember 31, 2023muta abiff, Mohammad Alshebremi, Melissa Bonner, Jay T. Myers, Byung-Gyu Kim, Suzanne L. Tomchuck, Alicia Santin, Daniel Kingsley, Sung Hee Choi, Alex Y. Huanghours prior to staining cells were incubated with BioLegend Cell Activation Cocktail with Brefeldin A (#423304). Cells were washed in FACS buffer prior to acquisition. Where appropriate, the number of events of

(2) LFA-1 regulated by IL-2/STAT5 pathway boosts antitumor function of intratumoral CD8+ T cells for improving anti-PD-1 antibody therapyOncoimmunologyDecember 21, 2023Jiqi Shan, Wei Jing, Yu Ping, Chunyi Shen, Dong Han, Fengsen Liu, Yaqing Liu, Congcong Li, Yi ZhangTo detect cytokine production, cells were treated with a Cell Activation Cocktail (BioLegend, cat. #423304) for 4 h. The cells were stained with anti-CD45 antibody (BioLegend, cat. #103108), anti-CD8a (BioLegend,

(3) Antigen recognition reinforces regulatory T cell mediated Leishmania major persistenceNat CommunDecember 19, 2023Romaniya Zayats, Zhirong Mou, Atta Yazdanpanah, Gaurav Gupta, Paul Lopez, Deesha Nayar, Wan H. Koh, Jude E. Uzonna, Thomas T. Murookaexperiments, the cells were stimulated with Cell activation cocktail with Brefeldin A (BioLegend Cat #423304) and incubated at 37 °C for 4 h prior to flow cytometry staining for IL-10 and/or IFNγ. Phenotypic characterization

(4) Hookworms dynamically respond to loss of Type 2 immune pressurePLoS PathogDecember 11, 2023Annabel A. Ferguson, Juan M. Inclan-Rico, Dihong Lu, Sarah D. Bobardt, LiYin Hung, Quentin Gouil, Louise Baker, Matthew E. Ritchie, Aaron R. Jex, Erich M. Schwarz, et al.S1 Table. MLN cells were stimulated with Cell Activation cocktail with Brefeldin A (Biolegend, Cat#. 423304) for 5–6 hours, followed by live dead staining using the LIVE/DEAD Fixable Aqua Dead Cell Stain Kit, for 405 nm excitation

(5) Detection of the CD8+ T cell immune response in mice infected with OVA-Listeria monocytogenesSTAR ProtocolsDecember 1, 2023Yudai Xu, Zijian Wang, Wen Gao, Haoyun Wang, Zhixian Wang, Chenxuan Tian, Guobing Chen(BHI),Oxoid,Cat# CM1135 Agar,Biosharp,Cat# BS195-1k Cell Activation Cocktail (with Brefeldin A),BioLegend,Cat# 423304 10× Intracellular Staining Perm Wash Buffer,BioLegend,Cat# 421002 Fixation buffer,BioLegend,Cat# 420801 RPMI

(6) Protocol for PPP1R15A-inhibited mouse model establishment with subcutaneous B16F1 tumor and single-cell analysisSTAR ProtocolsDecember 1, 2023Rongjing Wang, Minghui Wang, Siyu Pei, Yuchao Zhang, Shiwei Guo, Wei Guo, Zhenchuan Wu, Hailong Wang, Yizhe Li, Yufei Zhu, et al.before this procedure.a.Stimulate the cells with Cell Activation Cocktail with Brefeldin A (BioLegend, 423303) at a cell density of approximately 5 × 10ˆ6 cells/mL.Note: This step is performed to stimulate the

(7) Breast cancer stem cells generate immune-suppressive T regulatory cells by secreting TGFβ to evade immune-eliminationDiscov OncolDecember 1, 2023Sumon Mukherjee, Sourio Chakraborty, Udit Basak, Subhadip Pati, Apratim Dutta, Saikat Dutta, Dia Roy, Shruti Banerjee, Arpan Ray, Gaurisankar Sa, et al.with the cell activation cocktail (Ionomycin and PMA) in presence of brefeldin-A (Biolegend, Cat. No. 423304) for the last 5 h at 37 °C to identify the intracellular cytokines TGFβ and IFNγ. The cells were then

(8) Ubc9 regulates the expression of MHC II in dendritic cells to enhance DSS-induced colitis by mediating RBPJ SUMOylationCell Death & DiseaseNovember 13, 2023Jing Zhang, Longmin Chen, Qianqian Xu, Yuan Zou, Fei Sun, Qing Zhou, Xi Luo, Yang Li, Cai Chen, Shu Zhang, et al.intracellular cytokine staining, cells were first stimulated with Cell Activation Cocktail (with Brefeldin A) (423303; Biolegend, San Diego, CA, USA) for 4–6 h. The following antibodies were used for the studies: FITC anti-mouse CD11c

(9) Tumor-repopulating cell-derived microparticles elicit cascade amplification of chemotherapy-induced antitumor immunity to boost anti-PD-1 therapySignal Transduction and Targeted TherapyOctober 25, 2023Nana Bie, Tuying Yong, Zhaohan Wei, Qingle Liang, Xiaoqiong Zhang, Shiyu Li, Xin Li, Jianye Li, Lu Gan, Xiangliang Yang1:4. The CD3+ T cells were obtained and stimulated with cell activation cocktail (Biolegend, catalog #423304) containing phorbol 12-myristate-13-acetate (PMA, 40.5 µM), ionomycin (669.3 µM), and protein transport

(10) Uptake of long-chain fatty acids from the bone marrow suppresses CD8+ T-cell metabolism and function in multiple myelomaBlood AdvancesOctober 24, 2023Nancy Gudgeon, Hannah Giles, Emma L. Bishop, Taylor Fulton-Ward, Cristina Escribano-Gonzalez, Haydn Munford, Anna James-Bott, Kane Foster, Farheen Karim, Dedunu Jayawardana, et al.for cytokines were first stimulated for 4 hours with a cell-activation cocktail (BioLegend, catalog #423304). Cell culture supernatants from 0.2 × 106 cells were harvested and stored at −20°C for analysis of

(11) Global lactylome reveals lactylation-dependent mechanisms underlying T H 17 differentiation in experimental autoimmune uveitisScience AdvancesOctober 20, 2023Wei Fan, Xiaotang Wang, Shuhao Zeng, Na Li, Guoqing Wang, Ruonan Li, Siyuan He, Wanqian Li, Jiaxing Huang, Xingran Li, et al.and IFN-γ staining, cells were restimulated with Cell Activation Cocktail (Brefeldin A; BioLegend, 423304) for 6 hours at 37°C.The harvested cells were stained with surface marker with fluorescein isothiocyanate

(12) Subconjunctival injection of human umbilical cord mesenchymal stem cells alleviates experimental allergic conjunctivitis via regulating T cell responseStem Cell Research & TherapyOctober 2, 2023Dongli Li, Qingjian Ou, Qi Shen, Michael Mingze Lu, Jing-Ying Xu, Caixia Jin, Furong Gao, Juan Wang, Jingfa Zhang, Jieping Zhang, et al.Th2, cells were stimulated with a cell activation cocktail containing PMA, ionomycin, and Brefeldin A (423303, Biolegend) for 6 h. The cells were then fixed and permeabilized with Cyto-Fast™ Fix/Perm Buffer Set

(13) Myo9b mutations are associated with altered dendritic cell functions and increased susceptibility to autoimmune diabetes onsetNature CommunicationsSeptember 25, 2023Jing Zhang, Yuan Zou, Longmin Chen, Fei Sun, Qianqian Xu, Qing Zhou, Yi Wang, Xi Luo, Na Wang, Yang Li, et al.cocktails. For intracellular cytokine staining, cells were stimulated with Cell Activation Cocktail (423303; Biolegend, San Diego, CA, USA) for 4-6 h. Antibodies used are listed below: FITC anti-mouse CD11c (117306),

(14) Mechanism of Bazhen decoction in the treatment of colorectal cancer based on network pharmacology, molecular docking, and experimental validationFrontiers in ImmunologySeptember 20, 2023Shuai Lu, Xibo Sun, Zhongbao Zhou, Huazhen Tang, Ruixue Xiao, Qingchen Lv, Bing Wang, Jinxiu Qu, Jinxuan Yu, Fang Sun, et al.cytokine IFN-γ, the filtered cell suspension was stimulated with Cell Activation Cocktail (BioLegend, 423303) for 6 h. Then the cells were collected and stained for surface markers (44–48). Fixation Buffer (BioLegend,

(15) Palmitic Acid Upregulates CD96 Expression to Mediate Maternal–Foetal Interface Immune Tolerance by Inhibiting Cytotoxic Activity and Promoting Adhesion Function in Human Decidual Natural Killer CellsBioengineering (Basel)August 25, 2023Yingjie Wang, Yun Wangsuch as cytokines, we used Cell Activation Cocktail (with Brefeldin A) (Biolegend, San Diego, CA, USA, 423303) to stimulate the cells for 6 h prior to flow cytometry antibody staining of the cell surface. Fixation Buffer

(16) The correlation between Th17/Treg immune dysregulation and the disease severity in chronic spontaneous urticaria patientsImmunity, Inflammation and DiseaseJuly 27, 2023Xiaojing Yang, Leigang Chen, Shining Wang, Yuanhui Wu, Xiangzhao Zhou, Zhaoying MengTranscription Factor Buffer Se (424401), Permeabilization Wash Buffer (421002), and Cell Actibation Cocktail (423303) were obtained from BioLegend. 2.8 Enzyme‐linked immunosorbent assay (ELISA) for serum cytokine levels Serum

(17) Polysaccharides from Paecilomyces hepiali Prevent Acute Colitis in Association with Modulating Gut Microbiota and Treg/Th17 Immune Balance in MiceMoleculesJune 25, 2023Luwen Cui, Ningning He, Shengnan Yu, Hao Pang, Zixuan Zhang, Jingyi Wang, Jianhua Hao, Shangyong LiCell Activation Cocktail (with Brefeldin A, phorbol 12-myristate 13-acetate (PMA), ionomycin, etc.) (423303) and True-NuclearTM 4X Fix Concentrate (B337252) were purchased from BioLegend (San Diego, CA, USA). 4.2.

(18) Peripheral T cell profiling reveals downregulated exhaustion marker and increased diversity in lymphedema post-lymphatic venous anastomosisiScienceJune 16, 2023Hirofumi Imai, Takakazu Kawase, Shuhei Yoshida, Toshiro Mese, Solji Roh, Asuka Fujita, Toshio Uchiki, Ayano Sasaki, Shogo Nagamatsu, Atsushi Takazawa, et al.Factor Buffer Set,Bbiolegend,Cat#424401 Cell Activation Cocktail (with Brefeldin A),Biolegend,Cat#423303 Cyto-Fast Fix/Perm Buffer Set,Biolegend,Cat#426803 RNeasy Plus Universal Mini Kit,Qiagen,Cat#73404 Superscript III reverse transcriptase

(19) Single‐cell atlas reveals a distinct immune profile fostered by T cell‐B cell crosstalk in triple negative breast cancerCancer CommunicationsJune 1, 2023Shuning Ding, Niu Qiao, Qingchen Zhu, Yiwei Tong, Shengyue Wang, Xiaosong Chen, Qiang Tian, Yichuan Xiao, Kunwei Shenanalysis of cytotoxic CD8+T cells, cells were stimulated for 3 h at 37°C with a cell activation cocktail (423303, Biolegend), followed by staining with fixation/permeabilization buffer solution (BD Biosciences) according

(20) Vachellia farnesiana Pods or a Polyphenolic Extract Derived from Them Exert Immunomodulatory, Metabolic, Renoprotective, and Prebiotic Effects in Mice Fed a High-Fat DietInternational Journal of Molecular SciencesApril 28, 2023Claudia Delgadillo-Puga, Dulce R. Sánchez-Castillo, Yonatan Y. Cariño-Cervantes, Ivan Torre-Villalvazo, Claudia Tovar-Palacio, Sarai Vásquez-Reyes, Janette Furuzawa-Carballeda, Joshua Ayork Acevedo-Carabantes, María del Rayo Camacho-Corona, Jorge Luis Guzmán-Mar, et al.of cell activation cocktail with Brefeldin A (phorbol-12-myristate 13-acetate 40.5 mM BioLegend, Cat 423303) for 6 h. Cells were washed and resuspended with 1 mL of cold BD CytofixTM buffer (Cat. 554656) and

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3/21/2019Cell Activation Cocktail (with Brefeldin A)Dr. Javier CasaresAdding Cell Activation Cocktail (with Brefeldin A) stimulates cells to produce cytokines, such as IFNg.

Cell Activation Cocktail (with Brefeldin A) from BioLegend

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Cell Activation Cocktail (with Brefeldin A) from BioLegend

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